Catalog Number - 68304-05P-2

Cogent RP C4 Butyl™ is a reversed-phase HPLC stationary phase designed for the separation of hydrophobic to moderately hydrophobic compounds. Manufactured using a traditional butyl (C4) bonded phase on ultra-high purity silica and fully double end capped, it provides reproducible retention, excellent peak shape, and reliable performance for routine analytical methods. As a USP L26 equivalent phase, it is commonly used for method replacement, method development, and applications where less retention is desired than with C8 or C18 columns.

68304-05P-2 is an HPLC column packed with 3 µm RP C4 Butyl™ particles featuring a 100 Å pore size in a 2.1 mm ID x 50 mm format. This popular narrow-bore configuration is designed for rapid separations with reduced solvent consumption. The 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns, while the 50 mm length minimizes analysis times. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, HPLC systems must be capable of precise and reproducible low-flow operation, and detector flow cells should be appropriately configured for low-volume applications.

This column is particularly well suited for very hydrophobic and neutral compounds analyzed by reversed-phase chromatography. The combination of a short column length, narrow internal diameter, and lower-retention C4 chemistry makes it especially attractive for LC-MS applications where reduced solvent consumption, improved sensitivity, and fast analysis times are desired.

Key Benefits

• RP C4 Butyl™ stationary phase

• USP L26 equivalent chemistry

• Fully double end capped

• Traditional butyl (C4) bonded phase

• Ultra-high purity silica support

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Sharp peak shapes

• Fast analysis times

• Excellent for LC-MS applications

Typical Applications

• USP L26 methods

• Reversed-phase HPLC

• LC-MS methods

• Small molecule analysis

• Hydrophobic compound analysis

• Neutral compound analysis

• Pharmaceutical analysis

• Method development

• Quality control testing

• Fast separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C4 Butyl™

• USP Classification: L26

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Length: 50 mm

• Typical Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Suitable for conventional HPLC and LC-MS systems

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The 2.1 mm ID format reduces solvent consumption and is widely used for LC-MS applications

• Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.3 mL/min

• Detector flow cells should be optimized for low-volume, narrow-bore chromatography

• Ideal for rapid analyses and high-throughput workflows

• Lower retention than comparable C8 stationary phases

• Well suited for hydrophobic and neutral compounds

• Commonly used as an alternative for USP L26 methods

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